Insulin/Glucose Tolerance Test
Intraperitoneal injection or oral gavage administration of insulin or glucose
Insulin and glucose tolerance tests are vital tools for evaluating metabolic function in murine in vivo physiology, particularly in the context of metabolic diseases.
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Insulin tolerance tests gauge insulin sensitivity by monitoring blood glucose response to administered insulin, reflecting the animal's ability to utilize insulin effectively.
Glucose tolerance tests assess the ability to regulate blood glucose levels post-glucose load, offering insights into overall glucose metabolism.
These tests are commonly conducted following a 6-hour fasting period. Following either intraperitoneal (IP) injection or oral gavage administration, blood is sampled from the tail vein and blood glucose levels are determined via glucometer. The standard timeframe for these assessments is typically 120 minutes, providing a comprehensive understanding of the mouse's metabolic response.